FastQCFastQC Report
Tue 15 Mar 2016
G126-G127-M1M20-NEBNext21_GTTTCG-_AC8KUAANXX_L005_001.R1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext21_GTTTCG-_AC8KUAANXX_L005_001.R1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190172
Sequences flagged as poor quality0
Sequence length20-50
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC89308110.904300910896621No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC2032882.482096835084782No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1989622.4292774315362364No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC1779342.1725306867792273No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCC1158101.4140118180668244No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC830291.0137638135072133No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCC780460.9529226004044847No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGC504360.6158112430361659No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGC483120.589877721737712No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC472260.5766179269495195No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGC401170.48981877303675675No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCC375470.45843970065585926No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT272350.3325326989469818No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT249770.30496307037263687No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC249410.3045235191641885No Hit
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCC205660.25110583758192134No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCC198150.24193631098345675No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC175290.2140248092469853No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC159140.1943060536457598No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGC155860.19030125374656356No Hit
CGTCGGGGGCCCAAGTCCTTCTGATCGAGGCC152830.18660169774212312No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT151040.1844161514556715No Hit
CTAAACCATTCGTAGACGACCTGCTTC150780.18409869780512544No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC146260.17857988818794038No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG134690.1644532007386414No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG129690.15834832284352515No Hit
CCTGGGGGGCCCAAGTCCTTCTGATCGAGGCC117970.14403848905737265No Hit
TGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC117540.14351346955839267No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT112700.13760394775592016No Hit
GGTGGGGGCCCAAGTCCTTCTGATCGAGGCC111500.13613877706109223No Hit
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCC107650.13143802108185274No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCC107270.1309740503618239No Hit
TCTGTGACGTATGATGGATTCAATACATT107170.1308519528039216No Hit
CTCGCCCGTCACGTTGAACGCACGTTC102630.12530872367515603No Hit
GTCGGGGGCCCAAGTCCTTCTGATCGAGGCC102570.12523546514041464No Hit
NGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC96710.1180805482473384No Hit
CTAAACCATTCGTAGACGACCTGCTT96470.11778751410837282No Hit
GCTGAGAAGACGGTCGAACTTGACTATC93750.11446646053342958No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCC92930.11346526055863052No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCT91630.1118779923059003No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA90080.10998548015841425No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG88560.10812959727829893No Hit
GTAGGGGGCCCAAGTCCTTCTGATCGAGGCC88380.10790982167407472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG6800.0304.6858544
TCACCCG3200.0280.5648544
ATCACGC5550.0219.9241543
CGTAATA10650.0199.3335737
GTAATAT6150.0195.1818238
CCCGGGT30000.0189.7304134
TCACACG47600.0185.7132644
CGGCCCC47750.0183.7032630
GGCCCCG48900.0179.1845131
CCCCGGG48850.0178.2026833
GCCCCGG49750.0176.775732
CACAGGT7950.0176.6368644
TCGTAAT12400.0174.7998436
CACGCGT6500.0166.4574144
CACGTCT75800.0161.8738344
TTGCGGC48600.0160.7054627
TGCGGCC51050.0160.3917128
AGTCACG5500.0159.0521544
GGGGATT34500.0157.31427
GCCATAT15100.0151.8461330
Produced by FastQC (version 0.11.2)