FastQCFastQC Report
Fri 15 Apr 2016
G122-M5_GATCAG-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M5_GATCAG-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16532827
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC497000.300614045014806TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA68150.038.419911
ATCGGAA71700.036.5051772
TCGTATG70750.036.22546443
ACGATCA71900.035.82346332
ACACGTC73650.035.39005713
CGTATGC72450.035.31482744
CGATCAG73700.034.91879733
ACGTCTG75150.034.62272315
CTCGTAT71450.034.3915942
TCACGAT74850.034.3528130
AGTCACG75950.033.88341528
GTCACGA77250.033.39943729
CGTCTGA78400.033.01914616
CACGATC78000.032.93728631
CACGTCT82800.031.45101514
TCTCGTA73450.031.38671541
CACACGT83400.031.17813712
CGGAAGA86550.030.7010354
ATCTCGT79700.029.06296240
GCACACG90250.028.98610311
Produced by FastQC (version 0.11.2)