FastQCFastQC Report
Wed 2 Dec 2015
G122-M4_ACTTGA-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M4_ACTTGA-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909745
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2545021.345877482747652Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726770.3843362245233873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT390450.042.7584411
GATCGGA396300.042.438741
TCGTGTA392450.042.3932214
CGTGTAG394750.042.20752315
CGTCGTG396000.042.1437312
GTCGTGT398000.041.95031413
GTAGGGA397450.041.80499318
GTAGATC342900.041.76170331
AGCGTCG402700.041.47882510
AGAGCGT404350.041.4350248
TCTCGGT330600.041.34314736
TGTAGGG405750.041.2046817
GAGCGTC406900.041.0290539
ATCTCGG342550.040.82588235
GATCTCG339350.040.82243334
AAGAGCG410450.040.7334757
TAGGGAA410150.040.46761719
GTGTAGG414250.040.4388716
GAGTGTA393200.040.43166427
TGGTCGC308900.040.1012342
Produced by FastQC (version 0.11.2)