FastQCFastQC Report
Wed 2 Dec 2015
G122-M4_ACTTGA-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M4_ACTTGA-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909745
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3359241.7764597037136145TruSeq Adapter, Index 8 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377140.19944213948945375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA397350.043.2037051
ATCGGAA405800.042.242932
ACACGTC408450.042.1867813
TCGTATG406750.041.97181343
ACGTCTG412800.041.89963515
CTCGTAT392400.041.84574542
CGTCTGA413550.041.70642516
CGTATGC410650.041.54652844
CACACGT416150.041.42852412
CACGTCT418850.041.24781814
AATCTCG377500.040.95740539
TCTCGTA380500.040.70600541
CGGAAGA424300.040.4746864
GCACACG428750.040.18042411
ATCTCGT386450.040.10709840
TTGAATC394050.039.25301436
TCGGAAG440700.039.003553
GAATCTC399400.038.68948438
GAACTCC451000.038.26760521
CACTTGA448300.038.21696533
Produced by FastQC (version 0.11.2)