FastQCFastQC Report
Mon 23 Nov 2015
G122-M20_ATTCCT-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M20_ATTCCT-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17398335
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT16950.017.7806911
CGTCGTG17700.016.90371112
CGTGTAG19000.016.32697315
TCGTGTA20850.015.61693514
GATCGGA22700.013.5698821
GATCTCG20400.013.26443934
AGCGTCG24450.012.50622310
GTAGGGA25800.012.36505318
ATCTCGG23250.012.1114135
AGAGCGT26900.012.1033938
GAGCGTC28000.011.8634339
GTAGATC23950.011.75789631
CGATAGA7850.011.4900164
GTCGTGT27400.011.48201613
CCGATAG7800.011.2820513
TCTCGGT24700.011.2222236
ATCGGAA29000.010.7733742
GTGAGAT68350.010.1079951
TGTAGGG31800.010.1012117
GGTCGCC26350.09.85139843
Produced by FastQC (version 0.11.2)