FastQCFastQC Report
Mon 23 Nov 2015
G122-M19_ACTGAT-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M19_ACTGAT-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19378029
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTT380750.19648541139039477No Hit
GCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTT318100.16415498191276315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT244580.12621510680988246TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238060.12285047153144418No Hit
CCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCTAAAGTGTGTATT222590.11486720347048712No Hit
CTTGCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTT202620.10456171780938092No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC197080.10170280991941957No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA193820.10002049228020043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39200.033.595811
ATCGGAA40300.032.9946632
ACGTCTG46150.029.35661915
ACACGTC46350.029.27943413
CGTCTGA46300.028.92884316
CTCGTAT42750.028.5097144
CACACGT52150.026.11103612
GTGCCAG116950.025.0638521
TCTCGTA49450.024.46864743
TATCTCG50850.023.18683241
GCACACG58550.023.1484711
CGGAAGA62400.022.5449584
TGATATA58300.022.22227936
TCGGAAG64650.021.6923353
GATATAT57500.021.26930437
TGCCAGT140550.020.926062
CACGTCT68850.020.38071314
ACTGATA64950.020.25186234
ATATCTC58600.020.2327740
CTGATAT67550.019.3748835
Produced by FastQC (version 0.11.2)