FastQCFastQC Report
Mon 23 Nov 2015
G122-M18_GAGTGG-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M18_GAGTGG-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19554346
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA20700.022.7691571
ACGAGTG21650.020.72548132
ACACGTC26600.018.7700513
TCACGAG27750.017.20002630
ATCGGAA28000.016.9054242
CTCGTAT27400.016.45908244
CGAGTGG27800.016.37788833
ACGTCTG31800.015.83797315
AGTCACG30400.015.62780728
CGTCTGA31650.015.56546816
CACGAGT29150.015.54393431
TATCTCG30600.013.94591541
CACGTCT36450.013.75736314
CGGAAGA37700.013.7239764
AGTTACC57350.013.3981686
GTCACGA35750.013.16649129
CACACGT40450.012.67154812
GTTACCT62750.012.2100937
TCTCGTA36450.011.949965543
TACCTTG72300.010.7469819
Produced by FastQC (version 0.11.2)