FastQCFastQC Report
Mon 23 Nov 2015
G122-M17_CGTACG-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M17_CGTACG-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18085193
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG25600.025.00743512
GCGTCGT26250.024.80655711
CGTGTAG28350.022.1172315
TCGTGTA29300.021.7004714
GTCGTGT32250.020.2607213
GATCGGA31300.020.1753431
GTAGGGA32300.019.68532818
AGAGCGT34650.018.9831568
AGCGTCG35500.018.5905510
GTAGATC30150.018.46120631
GATCTCG29250.018.4270734
ATCGGAA34450.018.3935262
TGTAGGG38100.017.20832417
GTGTAGG39200.016.61318816
TCTCGGT32900.016.31550236
ATCTCGG33800.015.94623935
GAGCGTC42550.015.6654769
ATTTAGC49900.015.1684511
GAGTGTA41450.015.073812527
TAGGGAA44600.014.84837719
Produced by FastQC (version 0.11.2)