FastQCFastQC Report
Mon 23 Nov 2015
G122-M17_CGTACG-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M17_CGTACG-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18085193
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC19300.033.62067432
CCGTACG19550.032.8532133
GTACGTA21000.030.37579235
TACGTAA21100.029.8145136
CGTACGT21800.029.26084134
ACGTAAT21750.027.50766837
GATCGGA25100.026.3215751
CACCGTA25050.025.90335331
CGTAATC24900.023.58655238
ATCGGAA29750.022.1989672
ACACGTC30000.021.92227613
ACGTCTG32150.021.13899615
CGTCTGA34650.019.29633516
CTCGTAT32350.018.8374844
GTCACCG36350.018.27449829
CACACGT38550.017.34734312
CACGTCT40950.016.81151814
CGGAAGA43950.015.9288624
AATCTCG37950.015.7080441
TCGGAAG46750.015.3045113
Produced by FastQC (version 0.11.2)