FastQCFastQC Report
Mon 23 Nov 2015
G122-M16_GTTTCG-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M16_GTTTCG-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18823871
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT23600.020.88037311
CGTGTAG25850.018.89443415
TCGTGTA27300.018.21324314
CGTCGTG26900.017.82867812
GATCGGA28750.016.6847671
GATCTCG25900.015.71400634
AGAGCGT31900.015.3785878
GTAGGGA33000.014.73420618
GTCGTGT34650.014.5400813
GTAGATC29650.014.09796931
TCTCGGT30000.014.00600736
AGCGTCG35200.013.81170310
TGTAGGG37100.013.402410517
ATCGGAA36050.013.3661662
GAGCGTC37750.012.9370379
ATCTCGG31500.012.92019835
GTGTAGG39500.012.64378716
GGTCGCC34100.011.93460543
GTGGTCG36500.011.63214341
TGGTCGC37250.011.27970642
Produced by FastQC (version 0.11.2)