FastQCFastQC Report
Tue 16 Aug 2016
G122-M15_GTGGCC-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M15_GTGGCC-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20376418
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG40300.024.56544512
CGTGTAG40650.024.30070915
GATCGGA41400.024.0228521
GCGTCGT41600.024.00854311
GTAGGGA41550.023.88070718
TCGTGTA43200.023.63019814
GTCGTGT43800.022.90426813
GATCTCG35850.022.46007534
AGAGCGT46200.022.2372138
ATCTCGG39500.020.99709735
ATCGGAA47700.020.8029232
GTGTAGG49750.020.34259816
TGTAGGG50200.020.02876517
GTAGATC42850.019.76671631
TCTCGGT42850.019.5094336
GAGCGTC57750.017.637229
AGCGTCG59050.017.54688510
GGTCGCC45150.017.15065643
TAGGGAA60150.016.78876319
ATTTAGC61050.016.579011
Produced by FastQC (version 0.11.2)