FastQCFastQC Report
Mon 23 Nov 2015
G122-M14_GTGAAA-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M14_GTGAAA-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17059007
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT14850.017.48044411
CGTATGC14050.014.8775271
CGTCGTG18550.014.11294912
TCGTGTA20650.012.78513114
CGTGTAG21100.012.40819315
AGCGTCG23600.012.02467510
GATCGGA22000.012.001681
GTAGGGA23350.011.68372518
GTCGTGT23500.011.60877413
TGTAGGG26550.011.0213217
GATCTCG21750.010.62052634
AGAGCGT27250.010.4947598
GTGTAGG29150.09.96281216
GAGCGTC30800.09.713689
TTGCGCG9000.09.288318
GGTCGCC28350.09.07877343
ATCGGAA29350.09.0705582
ATCTCGG25900.08.74879735
GTCGCCG24450.08.727447544
TGGTCGC30550.08.49696642
Produced by FastQC (version 0.11.2)