FastQCFastQC Report
Mon 23 Nov 2015
G122-M14_GTGAAA-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M14_GTGAAA-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17059007
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16550.016.7673971
TGAAACG18500.014.50539535
AACGATC15350.014.18660538
CGTGAAA18750.013.72511233
GTCGGTT16750.013.6745051
ACGTGAA19100.013.58876332
GTCACGT21300.013.217875529
AGTCACG23300.012.74372628
ACACGTC22150.012.61104613
ACGTCTG22650.012.52613715
AAACGAT18700.012.23307937
ATCGGAA22600.012.1765752
GAAACGA21250.012.1105736
CGTCTGA23700.011.97107616
CTCGTAT22550.011.02433344
CACGTGA26050.010.55440131
ACGATCT22650.010.39123639
CTCCGTT24450.010.1787081
TCGGTTT22550.010.0557452
CGGAAGA32500.09.6195914
Produced by FastQC (version 0.11.2)