FastQCFastQC Report
Mon 23 Nov 2015
G122-M13_GTCCGC-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M13_GTCCGC-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18308790
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT25650.026.8438411
CGTCGTG28250.024.84155712
CGTGTAG31100.022.21364215
GATCGGA33050.021.3044031
GTAGGGA33250.020.91012618
TCGTGTA35250.019.72325114
AGCGTCG35600.019.64993910
GTCGTGT36450.019.37498513
AGAGCGT37800.019.263058
ATCGGAA37600.018.6083892
GTAGATC32500.018.07411631
GATCTCG30350.017.83154534
TGTAGGG39850.017.72302617
GTGTAGG43000.016.88521816
TCTCGGT35000.016.530536
GAGCGTC44200.016.4736489
ATCTCGG34500.016.0688435
TAGGGAA47600.014.74500519
GGTCGCC36700.014.38544643
GTCGCCG36700.014.02584844
Produced by FastQC (version 0.11.2)