FastQCFastQC Report
Wed 20 Apr 2016
G122-M3_CAGATC-_BC7TPAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M3_CAGATC-_BC7TPAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17112523
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC949970.5551314671717315TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA117850.041.0389021
CGTATGC120400.039.81355744
TCGTATG121300.039.5000243
ACACGTC124300.038.85913
ATCGGAA124600.038.7301452
CTCGTAT122650.037.9523142
ACGTCTG128200.037.70875515
CGTCTGA129000.037.45719516
CACACGT132200.036.65933212
CACGTCT133750.036.1609914
CGGAAGA136450.035.593964
TCTCGTA125450.035.27956841
TCGGAAG143000.033.9639053
GCACACG144100.033.71251711
ATCTCGT134150.033.02419340
ACCAGAT152800.031.30888732
CATCTCG141150.031.29174439
AGATCAT149650.030.32227935
AGTCACC161500.029.89414228
GAACTCC163100.029.78741821
Produced by FastQC (version 0.11.2)