FastQCFastQC Report
Wed 20 Apr 2016
G122-M2_GCCAAT-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M2_GCCAAT-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16624533
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1763441.0607455860564625Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT279300.042.34356311
GATCGGA282950.041.9912641
TCGTGTA282000.041.7787314
CGTCGTG283850.041.68869412
CGTGTAG283350.041.62626615
GTCGTGT285200.041.4245513
AGCGTCG286750.041.3118710
AGAGCGT289350.041.2225728
ATCGGAA289450.040.9403952
GTAGGGA287500.040.9193718
GTAGATC250200.040.7122831
GAGCGTC292600.040.56119
GATCTCG242450.040.478334
TCTCGGT239850.040.45762336
TGTAGGG294150.040.21867817
ATCTCGG247000.040.03474435
GAGTGTA281600.039.69650327
AAGAGCG299150.039.6810657
GTGTAGG299150.039.67884416
TAGGGAA297000.039.48456619
Produced by FastQC (version 0.11.2)