FastQCFastQC Report
Tue 28 Feb 2017
G121_M7M8M9.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M7M8M9.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78265850
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1817100.2321702249448514TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA605750.041.6831971
CTCGTAT581800.041.3778742
TCGTATG603750.041.3482343
CGTATGC603200.041.33392344
ATCGGAA610150.041.322962
TCTCGTA553300.041.0770241
CGTCTGA634250.040.2113616
ATCTCGT567550.039.9759540
ACACGTC638600.039.92354613
ACGTCTG647700.039.46801815
TCGGAAG657050.038.5380823
CACGTCT666750.038.3468914
CGGAAGA668950.037.75834
ACGATCA199800.036.8512732
CACACGT695100.036.7385712
GCACACG702550.036.29145411
CGATCAG203850.036.11549833
GTCACGA207000.036.0071329
AATCTCG200200.035.9719139
CACGATC205700.035.8905631
Produced by FastQC (version 0.11.2)