FastQCFastQC Report
Tue 28 Feb 2017
G121-M8_ACTTGA-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M8_ACTTGA-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26750286
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1499280.56047251233127TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA188850.041.718041
CTCGTAT181250.041.46735842
TCGTATG190050.041.19796843
TCTCGTA172950.041.19426341
CGTATGC191000.041.0267544
ATCGGAA192300.040.9605872
AATCTCG172300.040.81434639
ATCTCGT177700.040.05607640
CGTCTGA199900.039.9889116
ACACGTC201450.039.83391613
ACGTCTG205050.039.19887515
TCGGAAG206100.038.358363
CACGTCT212750.037.81125614
CGGAAGA209500.037.74054
CACACGT221000.036.4395812
GCACACG222850.036.07695411
TTGAATC224550.031.2880936
GTCACAC253250.031.16005329
GAACTCC258000.030.84594521
GAATCTC232300.030.41453638
Produced by FastQC (version 0.11.2)