FastQCFastQC Report
Tue 28 Feb 2017
G120-M9_CGTACG-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M9_CGTACG-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10819095
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT576110.5324937067287051TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG75550.041.9992433
CGTACGT75900.041.8621934
GATCGGA84200.041.57931
CGTAATC66850.041.44673538
CTCGTAT73650.041.25877844
ACCGTAC77000.041.12261232
GTACGTA77500.040.79164535
ATCGGAA85850.040.7282752
TCTCGTA68950.040.66109543
TACGTAA77000.040.52012336
CGTCTGA86350.040.4926516
CACCGTA78850.040.38346531
GTCACCG80150.040.3687329
ACGTAAT71200.040.14879637
AATCTCG69450.040.11673741
ACGTCTG88700.039.51688415
TCACCGT81150.039.51500730
ACACGTC88500.039.45701213
ATCTCGT71350.039.07927742
TCGGAAG90650.038.6258773
Produced by FastQC (version 0.11.2)