FastQCFastQC Report
Tue 28 Feb 2017
G120-M6_GTGAAA-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M6_GTGAAA-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960962
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG538480.41546298800968634Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG61050.041.22588744
AGCGTCG80700.040.97162610
CGTCGTG81200.040.9091412
GCGTCGT81400.040.7547311
GATCGGA82750.040.4944041
ATCGGAA83050.040.2429352
TGGTCGC61800.039.9061742
TCGTGTA83750.039.66232714
GATCTCG68600.039.63850834
GTCGTGT85000.039.23464213
GTGGTCG64750.039.14133541
GAGCGTC84950.038.921849
CGTGTAG85600.038.85623615
GGTCGCC65700.038.3074843
TCGGAAG88050.037.98243
ATCTCGG72600.037.90910735
AGAGCGT87550.037.8413478
TCGGTGG73950.037.7228138
TCTCGGT71200.037.60395436
CGGTGGT71100.037.25415839
Produced by FastQC (version 0.11.2)