FastQCFastQC Report
Tue 28 Feb 2017
G120-M13_CGATGT-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M13_CGATGT-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57733031
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG60150.039.16927312
GCGTCGT60750.038.7824511
AGCGTCG61300.038.7213110
GTCGCCG48250.037.662244
TGGTCGC56800.031.8372242
GAGCGTC74650.031.8260969
GTCGTGT75200.031.68042613
GATCGGA76050.031.5664141
GGTCGCC60100.030.63815543
GATCTCG65500.030.6317634
TCGTGTA78550.030.16118614
GTGGTCG61450.030.00086441
CGTGTAG80700.029.22121615
ATCGGAA83750.028.9017732
AGAGCGT85050.028.1671548
AAGAGCG89100.027.0124557
ATCTCGG78750.025.78514335
TCGGTGG78850.025.47334338
TCGGAAG95300.025.30633
TCTCGGT79850.024.63094136
Produced by FastQC (version 0.11.2)