FastQCFastQC Report
Tue 28 Feb 2017
G120-M10_ACTGAT-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M10_ACTGAT-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12471057
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG273530.2193318497381577Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG43350.040.80121212
AGCGTCG43450.040.4035210
GTCGCCG31150.040.3989644
GCGTCGT44150.040.11171711
GATCGGA44500.039.6528551
ATCGGAA45450.038.7292252
GAGCGTC45600.038.595029
CGTGTAG46050.038.35841415
TCGTGTA46750.037.73716414
TGGTCGC33100.037.35369542
GTCGTGT47500.037.32655313
GTGGTCG34300.037.26552641
GGTCGCC33700.037.01505743
AGAGCGT48750.036.236428
GATCTCG36400.036.20249634
TCGGAAG49200.035.9559023
TCGGTGG39400.035.0650738
AAGAGCG50150.035.0081677
ATCTCGG38000.034.96764835
TCTCGGT38000.034.96764836
Produced by FastQC (version 0.11.2)