FastQCFastQC Report
Tue 28 Feb 2017
G120-M10_ACTGAT-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M10_ACTGAT-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12471057
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT308770.24758927811812584TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA44250.039.5585981
CTCGTAT38050.039.2947344
TCTCGTA36600.038.1496243
ATCGGAA46300.037.927352
TATCTCG37100.037.6375941
CGTCTGA47050.036.85313816
ACACGTC47700.036.2562113
ACGTCTG48350.036.13315
ATCTCGT39350.035.59726742
TCGGAAG49550.035.399873
CGGAAGA51700.033.765664
CACGTCT52300.033.2776814
GCACACG54600.031.7906811
CACACGT57050.030.50341812
CGACGCT2650.024.08038917
GAACTCC73500.023.8980821
GTCACAC68000.023.79134229
ACTGATA69200.022.60630434
ACGACGC2950.022.37727216
CACGACG2950.022.37583415
Produced by FastQC (version 0.11.2)