FastQCFastQC Report
Sun 2 Apr 2017
G121_M1-M6.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M1-M6.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162581123
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5262550.043.310121
ATCGGAA5276600.043.047962
CGTCTGA5368600.042.6475616
ACACGTC5380600.042.63250713
CGTATGC5283750.042.62663744
TCGGAAG5359300.042.5114823
CTCGTAT5162250.042.4965442
TCGTATG5301100.042.4900943
ACGTCTG5400500.042.4802615
TCTCGTA4863850.042.4544541
CGGAAGA5381150.042.3131454
CACGTCT5448850.042.1396914
ATCTCGT4891950.042.13915640
TATCTCG2545550.041.6929139
CACACGT5520650.041.62510712
GCACACG5522750.041.53759811
CGATGTA1609350.041.45121434
CGCCAAT1115150.041.1363633
CACCGAT1645900.041.07497431
ACCGATG1645900.040.923932
Produced by FastQC (version 0.11.2)