FastQCFastQC Report
Sun 2 Apr 2017
G121-M1_ATCACG-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M1_ATCACG-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25889137
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC979230.3782397227068635TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG131700.039.83179543
GATCGGA133100.039.7145271
CTCGTAT127550.039.5111542
TCTCGTA120450.039.357541
CGTATGC134300.038.99450744
ATCGGAA136300.038.7231832
CACGATC120400.038.66205236
ACGATCT120850.038.5713337
ATCACGA133300.038.19294734
TCACGAT124900.038.1620235
CGTCTGA140550.037.98046516
GATCTCG124300.037.8739239
CATCACG135850.037.72224833
CGATCTC124450.037.6339238
ATCTCGT126250.037.46230740
ACACGTC143200.037.29276313
ACGTCTG145850.036.8263915
TCGGAAG149950.035.3852583
CACGTCT154200.034.77514614
CGGAAGA154350.034.1956374
Produced by FastQC (version 0.11.2)