FastQCFastQC Report
Sun 2 Apr 2017
G121_M7-M12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M7-M12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156113455
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1818170.1164646570662343TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1365300.041.985721
ATCGGAA1374850.041.582152
CTCGTAT1321050.041.43132442
TCGTATG1365000.041.37901743
CGTATGC1365850.041.35076544
TCTCGTA1258700.041.11300741
CGTCTGA1421600.040.62135316
ACACGTC1430650.040.38128313
ATCTCGT1286900.040.1438240
ACGTCTG1448900.039.99109315
TCGGAAG1465250.039.1820953
CACGTCT1491900.038.82665614
CGGAAGA1487800.038.5204664
AATCTCG574550.037.7943739
CACACGT1541050.037.5483712
GCACACG1554850.037.1983411
TATCTCG288300.033.0416439
GAACTCC1761800.032.6687421
ACGATCA230350.032.04011532
CATCTCG434950.031.95181739
Produced by FastQC (version 0.11.2)