FastQCFastQC Report
Thu 6 Aug 2015
G119-M3_AGTCAA-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M3_AGTCAA-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19068542
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2614111.3709018759798206TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG324600.053.4846748-49
CGTATGC331250.052.635846-47
TCGTATG334750.052.38603644-45
CTCGTAT323150.052.3517744-45
ATGCCGT348900.049.93865248-49
AATCTCG329900.049.00835440-41
GCCGTCT355600.048.8885450-51
TGCCGTC355600.048.6961350-51
TCTCGTA335350.048.61182842-43
ATCTCGT335100.048.23973542-43
CCGTCTT367600.046.7743252-53
GTATGCC395850.044.57205646-47
CGTCTTC397950.043.5957552-53
AGTCAAC438250.039.79135534-35
GCTTGAA444250.038.9146760-61
TGCTTGA453600.037.7714760-61
AACAATC429550.037.63895438-39
GTCAACA477850.036.59963234-35
CAGTCAA499000.035.2123332-33
CAACAAT473700.035.08521736-37
Produced by FastQC (version 0.11.2)