FastQCFastQC Report
Thu 6 Aug 2015
G119-M14_ATTCCT-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M14_ATTCCT-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19544546
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG364840.18667100274419268No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG314200.1607609611397471Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG262480.13429833570961433No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG204540.10465323676487548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT86800.052.0282867
TCTCGAC24950.032.6714638
GTAGCGA39500.031.1811288
GCCGAGA122500.030.7458278
AGTAGCG39900.030.2719637
TAGCGTC76950.029.73062976-77
ACTCTCG28300.029.6448366
TATACGC66750.028.702581110-111
CGGTAGT41750.028.6454663
TACGCTA65800.028.529215112-113
AAACCCG74400.027.590687108-109
ATCGGTA44700.026.3556671
CGCTATT67900.026.288622114-115
ACGAGCT88600.025.2505382-83
GCGTAGC85300.024.79744374-75
ACTCGTC87650.024.70960664-65
CTACGAG93550.024.55047280-81
CGGCGTA87300.024.5019772-73
GTAGCGT88850.023.84014576-77
TACGAGC77350.023.38458382-83
Produced by FastQC (version 0.11.2)