FastQCFastQC Report
Thu 6 Aug 2015
G118-M9_CGTACG-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M9_CGTACG-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906474
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA429286987.49397224972557No Hit
CTCTTTGTCTCCCATTAACCATACTTACCAGATCGGAAGAGCGTCGTGTA289940.5909335298627895Illumina Single End PCR Primer 1 (100% over 21bp)
CTCTTTGTCTCCCATTTTAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA74020.15086190205022995No Hit
CTCTTTGTCTCCCCTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA70250.1431781764256776No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGCAGCA56080.1142979663196014No Hit
CTCTTTTTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA54420.11091468129658896No Hit
CTCTTTGTCTCCCGTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA54250.11056820030025634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCC4657650.0118.91557
TTGTCTC4697000.0118.890895
TCTTTGT4715250.0118.855972
TGTCTCC4677450.0118.8509756
TCTCCCA4644050.0118.8435068
CTCCCAT4639350.0118.8330849
CTTTGTC4702150.0118.8315353
CTCTTTG4702950.0118.807521
TTTGTCT4716200.0118.776524
CTCCCGT10200.0110.834349
TCTCCGA3600.0110.737128
TCTCCCG9900.0110.5868768
CGCCCAT2100.0110.5010159
GTCTCCG4050.0110.1861957
GTCTCGC4200.0109.0843357
CTCCGAT3750.0106.307649
CGTCTCC1850.0106.136116
GTCTCCT11150.0105.660167
TCTCCCC20800.0104.125958
TCTCCTA10500.0102.5676048
Produced by FastQC (version 0.11.2)