FastQCFastQC Report
Thu 6 Aug 2015
G118-M3_ATGTCA-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M3_ATGTCA-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6306223
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG93381014.807754181861313No Hit
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC84111613.337872764727793No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT77677412.317579000932888No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA66755410.585638979148056No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT63859710.126457627648119No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA5092048.074627237254376No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC2916334.624527232861889No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG858371.3611475521877359No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG418610.6638046260019667No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGGATCAAGAGCTTAAAGAT118450.18783033838162716No Hit
TTTTAAAATCAGCCAGATGGTCAGGAGGAGCAGGTGGAAGCCAGATGGGA63280.10034532556175066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT614000.0118.1084758
CAGTTAC903950.0118.032414
TCAGTTA905200.0117.935163
GTGTATC613800.0117.9249
AGGTGTA613050.0117.893567
GAACCTT946850.0117.887744
GTCAGTT904800.0117.855782
TTAGTAT952700.0117.8508459
CCTTAGT953250.0117.74547
CTTAGTA951600.0117.736968
GTTACAA909800.0117.70516
AATTCTC1036250.0117.610483
TGGTTGA989150.0117.592439
TAAATTC1037600.0117.457451
TTACAAT915500.0117.414197
AGTTACA911600.0117.348675
ATCAGCC737550.0117.322018
ACAATGT910200.0117.228469
ATGGTTG989950.0117.022588
AAGAACC948550.0117.005272
Produced by FastQC (version 0.11.2)