FastQCFastQC Report
Thu 6 Aug 2015
G118-M2_AGTTCC-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M2_AGTTCC-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6005478
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG100329116.70626384777365No Hit
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC76824012.792320611281898No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT73126912.176699340169092No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA62583110.421002291574458No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA5290738.809839949459477No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT4839338.058192869909773No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC3359045.593293323195922No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG1062971.7700006560676769No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG607171.011026932410709No Hit
TTTTAAAATCAGCCAGATGGTTCAAAAGTAGCTATGGACCTGGGGTCTCC96590.16083648961831182No Hit
TTTTAAAATCAGCCAGATGGTTCAAAAGTAGCTATGGACCCGGGGTCTCC93990.15650710900947434No Hit
TTTTAAAATCAGCCAGATGGTCAGGAGGAGCAGGTGGAAGCCAGATGGGA75320.12541882594524534No Hit
TTTTAAAATCAGCCAGATGGACTCAGAGAAAAAAACAAAACAAAACAAAA67070.11168136824412644No Hit
TTTTAAAATCAGCCAGATGGGCCAGGCAGAGACAGACAGCATGAATGTTT63110.1050873885475894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATC627500.0118.108659
CAGTTAC839500.0117.9874344
TTAGTAT869650.0117.939499
GGTGTAT630150.0117.904678
TAAATTC1106050.0117.865871
TCAGTTA840650.0117.811873
GTTACAA842850.0117.806936
CCTTAGT869200.0117.747287
CTTAGTA870650.0117.5998
GAACCTT867850.0117.568034
ATCAGCC811450.0117.540798
GTCAGTT841300.0117.494522
TGGTTGA801850.0117.463949
GGTCAGT837200.0117.458721
AGTTACA845300.0117.4443745
AAGAACC864650.0117.404462
AATTCTC1109000.0117.4021153
AGGTGTA632300.0117.240287
TTCAGGT638150.0117.014964
GTTTCAG642350.0116.963112
Produced by FastQC (version 0.11.2)