FastQCFastQC Report
Thu 6 Aug 2015
G118-M1_AGTCAA-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M1_AGTCAA-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824844
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG108093115.838178865333772No Hit
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC101916514.93316184223405No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT80335911.771096892471096No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA77554211.363512484680967No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA5261987.710037035278755No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT5039047.383377554124314No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC4498926.591974849535022No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG981481.4380988048957604No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG644870.9448860662602692No Hit
TTTTAAAATCAGCCAGATGGTTCAAAAGTAGCTATGGACCTGGGGTCTCC129670.189997016781629No Hit
TTTTAAAATCAGCCAGATGGTTCAAAAGTAGCTATGGACCCGGGGTCTCC129220.1893376610513002No Hit
TTTTAAAATCAGCCAGATGGTCAGGAGGAGCAGGTGGAAGCCAGATGGGA92420.1354170146599688No Hit
TTTTAAAATCAGCCAGATGGACTCAGAGAAAAAAACAAAACAAAACAAAA87790.12863297681236377No Hit
TTTTAAAATCAGCCAGATGGGCCAGGCAGAGACAGACAGCATGAATGTTT76940.11273517753665871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATC625400.0118.210349
GTTACAA911400.0118.164376
CAGTTAC909600.0118.162714
TTAGTAT1145650.0117.976879
GAACCTT1139500.0117.971354
CCTTAGT1145750.0117.883487
TCAGTTA912250.0117.85863
GGTGTAT628450.0117.8165368
CTTAGTA1145900.0117.784988
TAAATTC1190400.0117.585481
AATTCTC1189650.0117.554573
GTCAGTT912900.0117.553072
AAGAACC1136150.0117.544112
AGTTACA916600.0117.442075
ATCAGCC992200.0117.4348458
GTCTCCC868250.0117.362167
AAATTCT1193200.0117.269652
GGTCAGT911050.0117.217061
AGGTGTA631400.0117.152997
TTACAAT921750.0117.082837
Produced by FastQC (version 0.11.2)