FastQCFastQC Report
Thu 6 Aug 2015
G118-M1_AGTCAA-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M1_AGTCAA-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824844
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG98123414.377383570965138No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA4858827.119312910302418No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT882001.292337231444411No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT510630.7481929257284123No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC421200.6171569635877392No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAATGATAGCACAT364670.5343272315088814No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG332230.4867950095269577No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT316500.46374686366457607No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC215690.3160365277213662No Hit
GAAGCAGTAGCAGAGTGATCAATGGAATGCGGGCTGTGCCTTACTAAATG197990.2901018689951008No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG173380.25404243672089794No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA173240.25383730382701786No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA155070.227213984671298No Hit
ATATTTCAAGTGGCATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA83590.12247898999596181No Hit
ATATTTCAAGTGGCATGATCAAGTGACCATAGGTGTGTGGGTTCATTTCT83490.12233246650033319No Hit
CACACTGCTCTTAGAGGATCTGCAACCCTATAGGTGGAACAACATTATGA80030.11726275355158301No Hit
ATATTTCAAGTGGCATGATCTTCAACCCTATAGGTGGAACAACATTATGA78140.11449345948420214No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA71600.10491082287009051No Hit
CACACTGCTCTTAGAGGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA69580.10195104825839242No Hit
GAAGCAGTAGCAGAGTGATCCTGCATCTAATGTCCCCCAGCCTCCAAATT68990.10108655963418359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT802100.0117.0719451
GTTTAGA1176150.0117.049397
AAGTGGC1135600.0116.995758
GTATGGT874350.0116.984536
GCAGTAG957600.0116.9692154
AGGTTTA1129200.0116.8834845
GAGGTTT1111000.0116.851734
TGAGGTT1117300.0116.5381243
AGTAGCA1011600.0116.486416
TATGGTT878150.0116.4105457
AGTGGCA1140950.0116.353299
GTAGCAG1013300.0116.202917
CAAGTGG1144000.0116.0950857
TTTGAGC811250.0115.9923256
ACACTGC882850.0115.853962
TGAGCTC809950.0115.664318
CTCTTAG928750.0115.5646448
TAGCAGA1017650.0115.524968
ATGGTTA886550.0115.522318
CATGAGG1127300.0115.425831
Produced by FastQC (version 0.11.2)