FastQCFastQC Report
Thu 6 Aug 2015
G118-M17_ACAGTG-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M17_ACAGTG-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25687682
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2840301.1057050612819017Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC809600.064.159699
AGAGCGT842550.061.6788068
AAGAGCG886100.058.8085757
CGCCGTA314300.056.04508246-47
GCCGTAT334550.054.0755346-47
CGTATCA333600.053.5250148-49
CCGTATC340400.052.9626748-49
TCGCCGT327550.052.397444-45
GATCTCG420300.051.77050434-35
ATCTCGG443100.049.2006134-35
CGGAAGA1073150.048.607964
TCTCGGT438450.048.35178436-37
GTCGCCG390300.048.34087444-45
GATCGGA1119200.046.78341
ATCGGAA1116900.046.719922
GTATCAT379700.046.54850850-51
TCGGAAG1133450.046.3999523
TAGATCT479950.046.35283332-33
GTAGATC485300.046.02573830-31
GTGGTCG411550.046.0183340-41
Produced by FastQC (version 0.11.2)