FastQCFastQC Report
Thu 6 Aug 2015
G118-M16_TGACCA-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M16_TGACCA-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29684228
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC8869642.9879975318879777TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC2138150.064.7959448
GAGCACA2160500.064.296389
CGGAAGA2237800.059.109064
AAGAGCA2370000.058.4747247
TATGCCG1173900.056.61643246-47
GAAGAGC2468200.056.2832346
ATCGGAA2361500.055.9340442
CTCGTAT1147000.055.8952142-43
TCGGAAG2375100.055.8792843
CGTATGC1222700.054.78214644-45
ATGCCGT1223450.054.35263446-47
GATCGGA2440850.054.333361
GCCGTCT1221400.054.11554748-49
AATCTCG1109050.053.4731938-39
ATCTCGT1109050.053.36316740-41
GGAAGAG2627600.052.2661445
TGCCGTC1267800.051.85809348-49
TCGTATG1306650.051.75460442-43
CCGTCTT1271200.051.11491850-51
ACCAATC1163100.050.3395736-37
Produced by FastQC (version 0.11.2)