FastQCFastQC Report
Thu 6 Aug 2015
G118-M13_ATCACG-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M13_ATCACG-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22260371
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5140522.309269688272491TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG708550.055.71111746-47
CTCGTAT696450.054.35439742-43
CGTATGC745900.053.267644-45
GCCGTCT745400.052.29878248-49
ATGCCGT751200.052.10452746-47
GATCTCG667750.051.48688538-39
ACGATCT665650.051.25684436-37
TCGTATG777300.051.14635542-43
ATCTCGT673950.050.99557540-41
TGCCGTC775600.050.41965548-49
TCACGAT707950.049.95858834-35
ATCACGA789900.049.3555234-35
CCGTCTT790200.048.99424450-51
CACGATC700400.048.84543236-37
CTTGAAA805800.047.0919160-61
GAGCACA1786000.046.862799
CGTCTTC833100.046.349950-51
GCTTGAA830750.046.21093458-59
AGAGCAC1807150.046.169468
CATCACG859200.046.1561132-33
Produced by FastQC (version 0.11.2)