FastQCFastQC Report
Thu 6 Aug 2015
G118-M10_GAGTGG-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M10_GAGTGG-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782242
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA331424987.62657175294441No Hit
CTCTTTGTCTCCCATTAACCATACTTACCAGATCGGAAGAGCGTCGTGTA316480.836752381259581Illumina Single End PCR Primer 1 (100% over 21bp)
CTCTTTGTCTCCCATTTTAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA61370.16225825846151568No Hit
CTCTTTGTCTCCCCTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA56530.1494616156237491No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTTGGCCTTGGCATGGAAGCA49290.13031953005651145No Hit
CTCTTTTTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA47040.12437067749763236No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGTGCCTTGGCATGGAAGCA41840.1106222182504451No Hit
CTCTTTGTCTCCCGTTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA41420.10951176577278768No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGCAGCA41330.1092738116704325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCC3616750.0118.9062357
TGTCTCC3630250.0118.8918156
TCTTTGT3654250.0118.869752
TCTCCCA3605250.0118.864678
CTTTGTC3645400.0118.8596343
TTGTCTC3645250.0118.854745
TTTGTCT3657500.0118.816184
CTCTTTG3646700.0118.810741
CTCCCAT3605450.0118.7871259
CTCCCGT8700.0113.528739
TCTCCCG8700.0110.79318
TGTCTCG3950.0108.4556966
TCTCCTA8150.0108.049088
GTCTACC6000.0107.099997
GTCTCCT8950.0106.368727
GTCTCGC3900.0105.2692267
GTATCCC4600.0103.4782647
TCTCCCC16800.0103.062518
GTTTCCC6700.0103.014937
TCGTTGT1800.0102.472232
Produced by FastQC (version 0.11.2)