FastQCFastQC Report
Mon 26 Sep 2016
G119-M9_GTGGCC-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M9_GTGGCC-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18927529
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC1100980.5816818455277496No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG1065900.5631479946484298No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1046930.5531255559032561No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC987840.5219064781250633No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC838910.4432221448452146No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT752350.3974898149673949No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT747740.3950542091363326No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG738720.39028866367078346No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA624970.33019101436854226No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG613870.32432654045860926No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC533820.28203364527931774No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA529230.27960860606791305No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC514240.2716889246345891No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC492780.260350941742052No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT488300.25798401893876377No Hit
ATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCG487550.2575877707016061No Hit
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG476220.25160178066561145No Hit
CTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAA434540.2295809452993045No Hit
CCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGG415320.21942642380841154No Hit
GAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCC404670.21379969884077316No Hit
CGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAG392860.2075601099329976No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG372030.19655497555967288No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT364070.19234946093597322No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC346260.1829398861309366No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA343750.1816137753639157No Hit
CACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGG317600.16779792016168618No Hit
AAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGC303860.1605386524569583No Hit
AGAGCCCCTCGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGA302130.15962463985658137No Hit
CCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG297150.15699355156185468No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA287780.15204309025229867No Hit
TCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTC285050.15060074666904485No Hit
CGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCC281090.14850855597685256No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT269540.14240633312462497No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG254180.13429116922763662No Hit
GTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCG242090.12790364764465556No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA239230.1263926210336278No Hit
TTCGACCGTCTTCTCAGCGCTCCGCCAGGCCGTGGGCCGACCCCGGCGGG237920.12570050744605912No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC233430.12332830133294209No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT223320.11798687509605718TruSeq Adapter, Index 20 (97% over 44bp)
AAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTAT221330.11693549644013225No Hit
GCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCA215070.11362814448732321No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGG205180.10840295106667121No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGC203800.10767385431030115No Hit
TGCCCCGGGCGTGGGGGGGGCGCGCGCCTCGTCCAGCCGCGGCGCGCGCC203580.10755762149406824No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACAT198080.1046518010882456No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT194950.10299812511184107No Hit
AAAGTGGCCCACTAGGCACTCGCATTCCACCCCGGCTCCACGCCAGCGAG190140.10045685308420343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT158150.083.625917
TCTCGAC76950.067.495718
ACTCTCG77450.067.444066
CTCGACT79850.065.342439
CGAAGGG105750.061.118911
CTCTCGA89050.058.4581037
GCATAGT282700.050.8021432
CCGGCCT282700.050.570653
AGTAGCG217900.050.51097
GGCATAG289100.050.173811
CGGTAGT222800.049.960783
GTAGCGA220550.049.877018
TTCGTGC25150.048.9669389
ATAGTTC293500.048.4868134
TAGCGAC229800.048.2576839
TCGGTAG231650.048.129122
ATGTCTC213900.047.1443332
TTAGAGC317950.045.7686589
TCACACC313450.045.7424439
AGTTCAC313600.045.3980566
Produced by FastQC (version 0.11.2)