FastQCFastQC Report
Mon 26 Sep 2016
G119-M8_GTGAAA-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M8_GTGAAA-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20462747
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1507740.7368218939519704TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG259600.040.46320748-49
CGTATGC274100.038.5506546-47
TCGTATG278400.038.2527444-45
CTCGTAT265600.038.1138344-45
GCCGTCT280350.037.66981550-51
ATGCCGT288150.036.90835648-49
CCGTCTT283500.036.90457552-53
TGCCGTC285500.036.8340150-51
TCTCGTA282500.034.0234142-43
GTATGCC315050.033.8231446-47
CGTCTTC319400.032.92421352-53
ATCTCGT289050.032.69667442-43
GATCTCG296100.032.1993340-41
AACGATC297450.031.53306238-39
ACGATCT304100.031.24457738-39
CGATCTC310000.030.4196940-41
GCTTGAA356000.029.55008560-61
AAACGAT333150.029.24302736-37
TGCTTGA369900.028.18228760-61
GAAACGA375300.027.74216536-37
Produced by FastQC (version 0.11.2)