FastQCFastQC Report
Mon 26 Sep 2016
G119-M4_AGTTCC-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M4_AGTTCC-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21713562
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3206731.4768327739133726Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA389300.049.61119548-49
CCGTATC397950.048.63749348-49
CGCCGTA403000.048.116646-47
GCCGTAT409250.047.6507446-47
TCGCCGT409050.047.10674744-45
GTCGCCG449000.045.40670444-45
GGTCGCC464800.043.77998442-43
TGGTCGC471850.042.92410342-43
GTATCAT447800.042.80452350-51
GTGGTCG488350.042.13174440-41
ATCATTA505550.039.50373552-53
CGGTGGT542200.038.9130138-39
TCTCGGT560700.038.16498636-37
ATCTCGG567300.038.0461134-35
TCGGTGG576850.037.9061838-39
GATCTCG570200.037.57608434-35
CTCGGTG577850.037.08377536-37
CATTAAA574450.035.68745854-55
TCATTAA578450.034.9623852-53
TATCATT559500.034.38121450-51
Produced by FastQC (version 0.11.2)