FastQCFastQC Report
Mon 26 Sep 2016
G119-M3_AGTCAA-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M3_AGTCAA-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19068542
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2423081.2707211699772327Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA274000.053.25671848-49
CCGTATC276950.052.79686448-49
CGCCGTA277400.052.41092746-47
TCGCCGT279300.051.5218144-45
GCCGTAT287900.051.10912346-47
GTCGCCG303950.050.0546644-45
GGTCGCC314000.048.26310342-43
TGGTCGC318050.047.47080642-43
GTGGTCG323250.047.39741540-41
GATCTCG349650.046.12451634-35
ATCTCGG353100.045.76653334-35
TCTCGGT354750.045.35239836-37
GTATCAT330200.044.28252850-51
CGGTGGT357150.044.2729638-39
TCGGTGG372200.043.77763738-39
CTCGGTG375050.042.74693336-37
GAGCGTC832600.041.8282289
GTAGATC410950.039.81618530-31
AGAGCGT877750.039.744138
ATCATTA386550.039.33563652-53
Produced by FastQC (version 0.11.2)