FastQCFastQC Report
Mon 26 Sep 2016
G119-M1_GGCTAC-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M1_GGCTAC-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19035836
Sequences flagged as poor quality0
Sequence length125
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1719370.9032279958705255No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG1093030.5741959533587072No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA914740.48053576422910976No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC712190.3741311912962478TruSeq Adapter, Index 11 (100% over 50bp)
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC524700.2756380124308698No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG470840.247344009477703No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG469960.24688172350297616No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA406640.21361814632149595No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC341600.17945101018941326No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGG331160.17396661748924502No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC321770.16903381600892128No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA297700.15638924395020004No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC292500.15365755409954152No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGG281010.14762157017952876No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC252270.13252373050492766No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC247620.13008096938847338No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC245480.12895677394993316No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC221190.11619663039753021No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG216290.11362253803825584No Hit
ATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAG213760.11229346586091621No Hit
CGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCC209750.11018691272608148No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG208020.10927810052576625No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG201010.10559557247709006No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGC200310.10522784499719372No Hit
GGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGG190790.1002267512706035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT245300.086.221217
CGGCCTC334550.063.3617028
GGCCTCC445100.047.985379
TATGCCG104950.044.16246-47
TGCGGCG508950.042.0238463
GCCGTCT116150.040.21077348-49
ATGCCGT116600.040.08125746-47
GTCGGAT160050.039.0457731
TGCCGTC120100.038.46718648-49
TCGGATT165950.037.6071362
CCTGCGG583850.037.0900421
TGTTACG51350.036.6115953
CTCGCTA44850.036.395051
TTACGAC51750.036.2136425
CTCGTAT134950.034.21196442-43
CATCTCG132400.033.5225138-39
GGGCGAT174200.032.61642536-37
TACGACT57800.032.3201836
ACGTTCG134950.032.226756
ACGGCTA147200.031.93334232-33
Produced by FastQC (version 0.11.2)