FastQCFastQC Report
Mon 26 Sep 2016
G119-M10_GTTTCG-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M10_GTTTCG-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267175
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG985940.44277731683520694Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG569310.2556723068822156No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG481450.21621512383137959No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG344380.15465814590310625No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC332760.14943970216248806No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG322050.14462993172685804No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT293450.13178591357008693No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC261820.11758114803516836No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC258510.11609465502471687No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA249030.11183726718813679No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT234160.1051592759297037No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT230090.1033314733458555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT97100.064.954277
TCTCGAC39450.053.8446168
ACTCTCG42300.051.0605356
AGTAGCG75100.046.110967
GTAGCGA75800.045.6066328
CTCTCGA48300.043.978687
CGGTAGT81050.042.8722273
ATCGGTA87600.039.66661
GCCGAGA204700.039.53148
CTCGACT57850.036.5132069
TCGGTAG96700.036.4875222
AGGGGGC225500.035.5943951
TAGCGTC99500.031.63361576-77
CTCGATC86050.031.42673130-31
CTACGAG158800.030.5555280-81
AAACCCG89200.030.550392108-109
GCATAGT130850.030.0114122
TATACGC122950.029.979816110-111
ACGAGCT160800.029.6389482-83
TACGCTA122550.029.56788112-113
Produced by FastQC (version 0.11.2)