FastQCFastQC Report
Mon 26 Sep 2016
G118-M18_GCCAAT-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M18_GCCAAT-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19421894
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1329780.6846809070217353Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA155700.053.67225646-47
GCCGTAT167550.051.61635246-47
CGTATCA168750.050.3854548-49
TCGCCGT164200.049.10015544-45
CCGTATC176450.048.97914548-49
GATCTCG214950.047.29271334-35
ATCTCGG229350.044.28447334-35
TCTCGGT224750.044.22455636-37
GTCGCCG201300.043.89341444-45
GAGCGTC568700.041.6407749
GTGGTCG215700.040.9079540-41
GTATCAT206350.040.85846750-51
TAGATCT255650.040.65966832-33
AGAGCGT596750.039.7034078
GTAGATC261750.039.65527730-31
GGTCGCC220050.039.62608342-43
CGGTGGT239250.039.36821438-39
AGTGTAG300800.037.45451428-29
TCGGTGG279150.037.31136738-39
AAGAGCG641000.037.111097
Produced by FastQC (version 0.11.2)