FastQCFastQC Report
Mon 26 Sep 2016
G118-M14_CGATGT-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M14_CGATGT-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27740869
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC7835472.8245221878233155TruSeq Adapter, Index 2 (100% over 50bp)
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC286080.10312582493360246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1628100.072.9701468
GAGCACA1652700.072.028019
CGGAAGA1616100.070.480594
ATCGGAA1677650.067.716492
TCGGAAG1715100.066.591683
GATCGGA1712250.066.509671
AAGAGCA1793200.066.202027
GAAGAGC1853650.064.2003566
GGAAGAG2021950.058.3381355
TATGCCG1006500.056.68081346-47
TATCTCG918550.056.60589238-39
CTCGTAT999600.055.9715342-43
CGTATGC1049650.054.62529444-45
ATGCCGT1055850.054.1752746-47
CGATGTA1041600.053.85893234-35
CACCGAT1069950.053.8225530-31
GCCGTCT1058100.053.79030648-49
ATCTCGT964200.053.45074540-41
TCGTATG1089100.053.31675342-43
TCACCGA1070950.053.31388530-31
Produced by FastQC (version 0.11.2)