FastQCFastQC Report
Mon 3 Jul 2017
G120-M3_ATGTCA-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M3_ATGTCA-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22786398
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2510651.10181960308075Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG289350.043.30073544
AGCGTCG376500.043.14954810
CGTCGTG377700.043.08205812
GATCGGA379850.043.0817831
GCGTCGT378150.043.05434811
ATCGGAA382400.042.7382622
TGGTCGC288350.042.6947242
GAGCGTC381200.042.490679
GTCGTGT383800.042.48707613
TCGTGTA382900.042.414514
GTGGTCG298700.042.38640241
GATCTCG319250.042.35231434
CGTGTAG384300.042.32867415
GGTCGCC294700.042.2749343
AAGAGCG386550.042.0485467
TCGGAAG388350.042.042973
AGAGCGT387350.041.9807368
ATCTCGG326000.041.94102535
TCGGTGG329650.041.93026438
TCTCGGT321800.041.68855736
Produced by FastQC (version 0.11.2)