FastQCFastQC Report
Mon 3 Jul 2017
G120-M2_AGTTCC-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M2_AGTTCC-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21628168
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1610510.7446354217333617Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG238150.042.9803810
CGTCGTG238950.042.9561712
GCGTCGT238700.042.94605611
GTCGCCG188700.042.9167644
GATCGGA242700.042.483351
ATCGGAA245050.042.0869642
GAGCGTC243250.042.024999
GTCGTGT244800.041.96325313
TGGTCGC189850.041.90267642
GATCTCG204800.041.76526334
TCGTGTA245500.041.76296214
GGTCGCC193400.041.74779543
GTGGTCG195500.041.62569441
CGTGTAG247750.041.4809615
AGAGCGT248250.041.338078
TCGGAAG250150.041.1759343
AAGAGCG250150.041.001317
CGGAAGA250450.040.8807034
ATCTCGG212400.040.83015435
TCGGTGG216700.040.6595538
Produced by FastQC (version 0.11.2)