FastQCFastQC Report
Mon 3 Jul 2017
G120-M2_AGTTCC-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M2_AGTTCC-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21628168
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1994400.9221308064557294TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA247250.042.6354831
CTCGTAT240700.041.8802544
ATCGGAA251350.041.748282
CCGTATC223750.041.4998338
TCCGTAT229500.041.29294237
TCTCGTA230600.041.27598643
CGTCTGA254750.041.230116
CGTATCT227450.041.09551239
AGTTCCG247500.041.0902334
ACACGTC257400.040.85266513
ACGTCTG258100.040.79358715
TATCTCG230750.040.6983841
GTTCCGT248950.040.61216735
TTCCGTA247850.040.6084936
ATCTCGT233600.040.56894342
TCGGAAG260100.040.4160923
CGGAAGA261150.040.060514
CACGTCT266550.039.5493514
CACACGT272750.038.68592512
GCACACG273200.038.612911
Produced by FastQC (version 0.11.2)