FastQCFastQC Report
Mon 3 Jul 2017
G120-M1_AGTCAA-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M1_AGTCAA-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21253759
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4795892.2564902519126147Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG545400.043.55251744
GATCGGA709000.043.322251
ATCGGAA709950.043.2008252
GCGTCGT707950.043.19423711
AGCGTCG707900.043.1941810
CGTCGTG708300.043.18200712
TGGTCGC540750.043.1368442
GGTCGCC548700.043.1172643
GTGGTCG556850.043.02350241
GATCTCG590000.042.97775334
GAGCGTC711500.042.938639
GTCGTGT715150.042.87166613
CGTGTAG712850.042.8679415
TCGGAAG717000.042.845733
TCGTGTA713650.042.81073814
ATCTCGG602350.042.7247835
AGAGCGT717950.042.666248
TCTCGGT590900.042.60736
TCGGTGG612250.042.59447538
AAGAGCG718250.042.582097
Produced by FastQC (version 0.11.2)