FastQCFastQC Report
Mon 3 Jul 2017
G117C_T5_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117C_T5_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39925875
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT80200.040.9709434
CCGTACG80800.040.802833
ACCGTAC86400.038.3363732
CTCGTAT95500.037.9508844
CGTAATC72350.037.57332638
GTACGTA87250.037.3831135
TCTCGTA89750.036.5749443
CACCGTA94200.035.348831
TACGTAA91500.035.16599736
ACGTAAT84350.034.54860337
GATCGGA118000.034.4859241
AATCTCG95150.034.2211941
GTCACCG100250.033.32522629
ATCTCGT98900.033.10194842
ATCGGAA124800.032.638562
TCACCGT103750.032.222230
CGTCTGA130100.031.78192716
CGTTTCG21000.030.68654833
ACACGTC135350.030.59790813
ACGTCTG135800.030.56129815
Produced by FastQC (version 0.11.2)